Mining RNAseq data reveals dynamic metaboloepigenetic profiles in human, mouse and bovine pre-implantation embryos

iScience

Marcella Pecora Milazzotto¹  Marcia de Almeida Monteiro Melo Ferraz²  Michael James Noonan³ 

[1] Center of Natural and Human Sciences, Federal University of ABC, São Paulo, 09210-580 Santo André, Brazil;Gene Center Munich, Ludwig-Maximilians University of Munich, 80539 Munich, Germany;The Irving K. Barber School of Sciences, The University of British Columbia, Okanagan Campus, Kelowna, BC V1V 1V7, Canada;

关键词: Biological sciences;    Molecular biology;    Endocrinology;    Omics;    Transcriptomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Metaboloepigenetic regulation has been reported in stem cells, germ cells, and tumor cells. Embryonic metaboloepigenetics, however, have just begun to be described. Here we analyzed RNAseq data to characterize the metaboloepigenetic profiles of human, mouse, and bovine pre-implantation embryos. In embryos, metaboloepigenetic reprogramming was species-specific, varied with the developmental stage and was disrupted with in vitro culture. Metabolic pathways and gene expressions were strongly correlated with early embryo DNA methylation and were changed with in vitro culture. Although the idea that the in vitro environment may influence development is not new, there has been little progress on improving pregnancy rates after decades using in vitro fertilization. Hence, the present data will contribute to understanding how the in vitro manipulation affects the metaboloepigenetic status of early embryos, which can be used to establish culture strategies aimed at improving the in vitro environment and, consequently, pregnancy rates and offspring health.

【 授权许可】

Unknown