Alleviating acute alcoholic liver injury in mice with Bacillus subtilis co-expressing alcohol dehydrogenase and acetaldehyde dehydrogenase

Journal of Functional Foods

Yunbin Lyu¹  Fengxia Lu²  Zhaoxin Lu²  Jing Sun²  Zhenghua Huang²  Jing Lu²  Mingtong Li² 

[1] Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China;Laboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, Jiangsu 210095, PR China;

关键词: Alcohol dehydrogenase;    Aldehyde dehydrogenase;    Bacillus subtilis;    Acute alcoholic liver injury;    Caecal microbiota;    Probiotic;   

DOI  :  

来源: DOAJ

【 摘 要 】

Alcohol consumption increases the risk of liver disease. Here, we investigated the effects of Bacillus subtilis co-expressing alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), key enzymes in ethanol degradation, on acute alcohol-induced liver injury. Recombinant B. subtilis significantly alleviated alcohol-induced increase in the liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, administration of ADH/ALDH-expressing B. subtilis inhibited lipid peroxidation and oxidative stress in the liver of alcohol-treated mice as evidenced by significant reduction of malondialdehyde and induction of total antioxidant capacity and glutathione and superoxide dismutase levels. Caecal microbiota diversity analysis indicated that administration of recombinant B. subtilis reversed alcohol-induced decrease in Firmicutes and increase in Proteobacteria, in particular pathogenic Helicobacter hepaticus, restoring the microbial composition in alcohol-treated mice. In conclusion, recombinant B. subtilis expressing ADH and ALDH could act as a novel candidate probiotic for prevention of alcohol-induced acute liver injury.

【 授权许可】

Unknown