Recombinant protein expression in Escherichia coli: advances and challenges

Frontiers in Microbiology

Eduardo A. Ceccarelli¹  Germán Leandro Rosano¹ 

[1] Facultad de Ciencias Bioquímicas y Farmaceúticas, UNR;Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET);

关键词: Escherichia coli;    Inclusion Bodies;    recombinant protein production;    expression plasmid;    affinity tags;    E. coli expression strains;   

DOI  :  10.3389/fmicb.2014.00172

来源: DOAJ

【 摘 要 】

Escherichia coli is the organism of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of recombinant proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.

【 授权许可】

Unknown