期刊论文详细信息
Frontiers in Microbiology | |
Recombinant protein expression in Escherichia coli: advances and challenges | |
Eduardo A. Ceccarelli1  Germán Leandro Rosano1  | |
[1] Facultad de Ciencias Bioquímicas y Farmaceúticas, UNR;Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); | |
关键词: Escherichia coli; Inclusion Bodies; recombinant protein production; expression plasmid; affinity tags; E. coli expression strains; | |
DOI : 10.3389/fmicb.2014.00172 | |
来源: DOAJ |
【 摘 要 】
Escherichia coli is the organism of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of recombinant proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
【 授权许可】
Unknown