Genome-Wide Quantification of the Effect of Gene Overexpression on Escherichia coli Growth

Genes

卷:9

Sumana Venkat¹  Hao Chen¹  Chenguang Fan¹  Paige McGuire²  Qinglei Gan³  Jessica Wilson⁴  AbigailL. Chang⁵ 

[1] Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA;

[2] Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA;

[3] Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA;

[4] Department of Chemistry, University of Arkansas, Fort Smith, AR 72913, USA;

[5] Fayetteville High School, Fayetteville, AR 72701, USA;

关键词: Escherichia coli;    recombinant protein production;    gene overexpression;    growth effect;    ASKA collection;    codon bias;    branched-chain amino acids;    gene ontology;   

DOI  :  10.3390/genes9080414

来源: DOAJ

【 摘 要 】

Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. Escherichia coli is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell growth has been much less studied. Here, we performed high-throughput tests on the E. coli a complete set of E. coli K-12 ORF archive (ASKA) collection to quantify the effects of overexpressing individual E. coli genes on its growth. The results indicated that overexpressing membrane-associated proteins or proteins with high abundances of branched-chain amino acids tended to impair cell growth, the latter of which could be remedied by amino acid supplementation. Through this study, we expect to provide an index for a fast pre-study estimate of host cell growth in order to choose proper rescuing approaches when working with different proteins.

【 授权许可】

Unknown