Antimicrobial Activity of Phenolic Compounds Extracted from Platanus hybrida: Exploring Alternative Therapies for a Post-Antibiotic Era

Proceedings

Alfredo Aires¹  Rosa Carvalho²  Gilberto Igrejas³  Patrícia Poeta⁴  Jessica Ribeiro⁴  Vanessa Silva⁴ 

[1] Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal;Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), 5001-801 Vila Real, Portugal;Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal;Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal;

关键词: polyphenols;    plant-derived compounds;    plane tree;    antimicrobial activity;    bacterial resistance;    public health;   

DOI  :  10.3390/proceedings2020066018

来源: DOAJ

【 摘 要 】

Multidrug-resistant bacteria are a significant threat to public health and new classes of antibiotics and approaches to treatment are needed. Several studies have shown that natural plant-derived compounds could be a promising mean to fight microbial resistance but only a few were conducted with antibiotic resistant bacteria. Therefore, the aim of this study was to extract phenolic compounds from the leaves, fruits, and tree trunk of Platanus hybrida and evaluate their antimicrobial activity against antibiotic resistant bacterial strains. The polyphenolic compounds were extracted using a water/ethanol (20:80) mixture. Two grams of powder of each sample was extracted with 100 mL of solvent by stirring for 2h. The extracts were redissolved in dimethyl sulfoxide (DMSO) to a final concentration of 100 mg/mL. An antimicrobial susceptibility assay was performed using the Kirby–Bauer disc diffusion method and was tested against ten different bacteria: Listeria monocytes, Bacillus cereus, Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. The fruits had the highest antibacterial activity showing a minimum inhibitory concentration (MIC) of 10mg/mL, contrary to the tree trunk that showed the lowest antibacterial activity. None of the extracts showed antimicrobial properties against S. enteritidis, E. faecium and E. faecalis. These results show that P. hybrida’s phenolic compounds act as antibacterial agents, which may become useful therapeutic tools and represent a source for the development of novel antimicrobials. However, they were not effective against all bacteria, which shows that polyphenols alone might not substitute antibiotics.

【 授权许可】

Unknown