Association of mitochondrial respiratory chain enzymes with the risk and mortality of sepsis among Chinese children

BMC Infectious Diseases

Guowei Song¹  Xiaodai Cui¹  Lipeng Zhang²  Qi Zhang²  Wenquan Niu³  Yuanmei Chen³  Danni He³  Linying Guo⁴  Wei Li⁴  Xiuxiu Lu⁴  Ning Li⁴  Zhongyuan Sun⁴ 

[1] Central Laboratory, Capital Institute of Pediatrics;Department of Pediatrics, China-Japan Friendship Hospital;Institute of Clinical Medical Sciences, China-Japan Friendship Hospital;Intensive Care Unit, Affiliated Children’s Hospital of Capital Institute of Pediatrics;

关键词: Sepsis;    Children;    Mitochondrial Respiratory Chain Enzymes;    Risk;    Mortality;   

DOI  :  10.1186/s12879-021-07014-6

来源: DOAJ

【 摘 要 】

Abstract Background Sepsis is a leading cause of pediatric morbidity and mortality worldwide. The aim of this study was to explore the association of decreased mitochondrial respiratory chain enzyme activities with the risk for pediatric sepsis, and explore their association with mortality among affected children. Methods A total of 50 incident cases with sepsis and 49 healthy controls participated in this study. The level of serum coenzyme Q10 was measured by high-performance liquid chromatography, and selected mitochondrial respiratory chain enzymes in WBC were measured using spectrophotometric. Logistic regression models were used to estimate odds ratio (OR) and 95% confidence interval (CI). Results The levels of CoQ10, complex II, complex I + III and FoF1-ATPase were significantly higher in healthy controls than in children with sepsis (p < 0.001, = 0.004, < 0.001 and < 0.001, respectively). In children with sepsis, levels of CoQ10 and complex I + III were significantly higher in survived cases than in deceased cases (p < 0.001). Per 0.05 μmol/L, 50 nmol/min.mg and 100 nmol/min.mg increment in CoQ10, complex I + III and FoF1-ATPase were associated with significantly lowered risk of having sepsis, even after adjusting for confounding factors (OR = 0.85, 0.68 and 0.04, p = 0.001, < 0.001 and < 0.001, respectively). Per 0.05 μmol/L and 50 nmol/min.mg increment in CoQ10 and complex I + III was associated with significantly lowered risk of dying from sepsis during hospitalization, and significance retained after adjustment (OR = 0.73 and 0.76, 95% CI: 0.59 to 0.90 and 0.64 to 0.89, p = 0.004 and 0.001, respectively) in children with sepsis. Conclusions Our findings indicate the promising predictive contribution of low serum CoQ10 and complex I + III to the risk of pediatric sepsis and its associated mortality during hospitalization among Chinese children. Trial registration The trial was registered with www.chictr.org.cn , number ChiCTR-IOR-15006446 on May 05, 2015. Retrospectively registered.

【 授权许可】

Unknown