Association of mitochondrial respiratory chain enzymes with the risk and mortality of sepsis among Chinese children

BMC Infectious Diseases

Xiaodai Cui¹  Guowei Song¹  Qi Zhang²  Lipeng Zhang³  Wenquan Niu⁴  Yuanmei Chen⁴  Danni He⁵  Xiuxiu Lu⁶  Linying Guo⁶  Zhongyuan Sun⁶  Wei Li⁶  Ning Li⁶ 

[1] Central Laboratory, Capital Institute of Pediatrics, Beijing, China;Department of Pediatrics, China-Japan Friendship Hospital, No.2 Yinghua East Street, Chaoyang District, 100029, Beijing, China;Department of Pediatrics, China-Japan Friendship Hospital, No.2 Yinghua East Street, Chaoyang District, 100029, Beijing, China;Graduate School of Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China;Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua East Street, Chaoyang District, 100029, Beijing, China;Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, No. 2 Yinghua East Street, Chaoyang District, 100029, Beijing, China;Department of Pediatrics, China-Japan Friendship Hospital, No.2 Yinghua East Street, Chaoyang District, 100029, Beijing, China;Intensive Care Unit, Affiliated Children’s Hospital of Capital Institute of Pediatrics, Beijing, China;

关键词: Sepsis;    Children;    Mitochondrial Respiratory Chain Enzymes;    Risk;    Mortality;   

DOI  :  10.1186/s12879-021-07014-6

来源: Springer

【 摘 要 】

BackgroundSepsis is a leading cause of pediatric morbidity and mortality worldwide. The aim of this study was to explore the association of decreased mitochondrial respiratory chain enzyme activities with the risk for pediatric sepsis, and explore their association with mortality among affected children.MethodsA total of 50 incident cases with sepsis and 49 healthy controls participated in this study. The level of serum coenzyme Q10 was measured by high-performance liquid chromatography, and selected mitochondrial respiratory chain enzymes in WBC were measured using spectrophotometric. Logistic regression models were used to estimate odds ratio (OR) and 95% confidence interval (CI).ResultsThe levels of CoQ10, complex II, complex I + III and FoF1-ATPase were significantly higher in healthy controls than in children with sepsis (p < 0.001, = 0.004, < 0.001 and < 0.001, respectively). In children with sepsis, levels of CoQ10 and complex I + III were significantly higher in survived cases than in deceased cases (p < 0.001). Per 0.05 μmol/L, 50 nmol/min.mg and 100 nmol/min.mg increment in CoQ10, complex I + III and FoF1-ATPase were associated with significantly lowered risk of having sepsis, even after adjusting for confounding factors (OR = 0.85, 0.68 and 0.04, p = 0.001, < 0.001 and < 0.001, respectively). Per 0.05 μmol/L and 50 nmol/min.mg increment in CoQ10 and complex I + III was associated with significantly lowered risk of dying from sepsis during hospitalization, and significance retained after adjustment (OR = 0.73 and 0.76, 95% CI: 0.59 to 0.90 and 0.64 to 0.89, p = 0.004 and 0.001, respectively) in children with sepsis.ConclusionsOur findings indicate the promising predictive contribution of low serum CoQ10 and complex I + III to the risk of pediatric sepsis and its associated mortality during hospitalization among Chinese children.Trial registration The trial was registered with www.chictr.org.cn, number ChiCTR-IOR-15006446 on May 05, 2015. Retrospectively registered.

【 授权许可】

CC BY