Malaria Journal | |
A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity | |
关键词: Anti-malarial; Assay; Haemozoin; Malaria; | |
DOI : 10.1186/1475-2875-11-272 | |
来源: DOAJ |
【 摘 要 】
Abstract
Background
The spread of drug resistance in malaria parasites and the limited number of effective drugs for treatment indicates the need for new anti-malarial compounds. Current assays evaluating drugs against
Methods
The relationship between parasitaemia and the haemozoin level of infected erythrocytes was investigated after converting haemozoin into monomeric haem. The 50% inhibitory concentration (IC50) values of chloroquine, quinine, artemisinin, quinidine and clotrimazole against
Results
The haemozoin of parasites was extracted and converted into monomeric haem, allowing the use of a colorimeter to efficiently and rapidly measure the growth of the parasites. There was a strong and direct linear relationship between the absorbance of haem converted from haemozoin and the percentage of the parasite (R2 = 0.9929). Furthermore, the IC50 values of drugs were within the range of the values previously reported.
Conclusion
The haemozoin-based colorimetric assay can be considered as an alternative, simple, robust, inexpensive and convenient method, making it applicable in developing countries.
【 授权许可】
Unknown