Genome-wide identification of neuronal activity-regulated genes in Drosophila

eLife

Reazur Rahman¹  Michael Rosbash¹  Xiao Chen²  Fang Guo² 

[1] National Center for Behavioral Genomics, Department of Biology, Brandeis University, Waltham, United States;Howard Hughes Medical Institute, Brandeis University, Waltham, United States;

关键词: RNA high-throughput sequencing;    neural firing;    optogenetics;    chromatin accessibility;    neural activity reporters;   

DOI  :  10.7554/eLife.19942

来源: DOAJ

【 摘 要 】

Activity-regulated genes (ARGs) are important for neuronal functions like long-term memory and are well-characterized in mammals but poorly studied in other model organisms like Drosophila. Here we stimulated fly neurons with different paradigms and identified ARGs using high-throughput sequencing from brains as well as from sorted neurons: they included a narrow set of circadian neurons as well as dopaminergic neurons. Surprisingly, many ARGs are specific to the stimulation paradigm and very specific to neuron type. In addition and unlike mammalian immediate early genes (IEGs), fly ARGs do not have short gene lengths and are less enriched for transcription factor function. Chromatin assays using ATAC-sequencing show that the transcription start sites (TSS) of ARGs do not change with neural firing but are already accessible prior to stimulation. Lastly based on binding site enrichment in ARGs, we identified transcription factor mediators of firing and created neuronal activity reporters.

【 授权许可】

Unknown