The Potential of a Surface-Modified Titanium Implant with Tetrapeptide for Osseointegration Enhancement

Applied Sciences

Yen-Chun Chuo¹  Ping-Jen Hou¹  Bai-Hung Huang¹  Keng-Liang Ou¹  Chia-Jen Wu²  Wen-Chien Lan³  Hsin-Yu Tsai⁴  Takashi Saito⁴  Ming-Liang Yen⁵  Syamsiah Syam⁶  Chung-Ming Liu⁶  Yu-Yeong Lin⁷ 

[1] Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan;Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan;Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, Taiwan;Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan;Division of Oral and Maxillofacial Surgery, Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan;Graduate Institute of Dental Science, College of Dentistry, China Medical University, Taichung 404, Taiwan;J.U.S.T Dental Clinic, Taipei 114, Taiwan;

关键词: tetrapeptide Gly-Arg-Gly-Asp;    osseointegration;    surface modification;    dental implant;   

DOI  :  10.3390/app11062616

来源: DOAJ

【 摘 要 】

In this study, the innovative dip-coating technique treated titanium (IDCT-Ti) implant with tetrapeptide Gly-Arg-Gly-Asp (GRGD) coating was investigated for its potential to enhance osseointegration. The L929 fibroblast cells were cultured in different concentrations of the GRGD (1%, 2%, and 5%). The cell viability was assessed through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and live/dead staining. The surface topography and nano-indentation were analyzed by atomic force microscopy. The hemocompatibility was evaluated via field-emission scanning electron microscopy, while contact angle analysis was detected by a goniometer. Radiograph evaluation was determined by panoramic imaging. It was found that the cell growth increased and had a survival rate of more than 70% in 1% GRGD. The mortality of L929 increased with the higher concentration of GRGD. The IDCT-Ti coated with 1% GRGD showed a nano-surface with a Young’s modulus that was similar to human cortical bone, and it displayed greater red blood cell accumulations with abundant fibrin formation. As regards the wettability, the IDCT-Ti coated with 1% GRGD was lower than the SLA (sandblasted, large-grit, and acid-etched) treated implant. The X-ray image exhibited no bone loss around the implant at six months after placement. As a result, this study suggests that the IDCT-Ti implant, coated with 1% GRGD, has a tremendous likeliness to enhance osseointegration.

【 授权许可】

Unknown