Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation

Parasite

关键词: Trypanosoma vivax;    cyclophosphamide;    isolation;    cryopreservation;    Percoll;    rodents;   

DOI  :  10.1051/parasite/2004111103

来源: DOAJ

【 摘 要 】

A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll solutions, 1.07, 1.06, 1.05 g/ml, respectively, carefully layered on top of each other. The yields of this simple procedure for trypanosome purification were about six times higher than those obtained with the conventional anion-exchange columns. Cryopreservation of trypanosomes using glycerol yielded 90 % viable parasites, whereas using dimethylsulfoxide, a more commonly used cryoprotectant, the viability was only 35 %.

【 授权许可】

Unknown