Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines

BMC Cancer

Xi Ren¹  Jin-Fen Xiao¹  Anand Mayakonda¹  H. Phillip Koeffler¹  Zhen-Tang Lao¹  Kar-Tong Tan¹  Liang Xu¹  Xin Yi Loh¹  Ling-Wen Ding¹  Qiao-Yang Sun¹  Henry Yang¹  De-Chen Lin²  Wenwen Chien²  Michael Lill² 

[1] Cancer Science Institute of Singapore, National University of Singapore;Division of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine;

关键词: BCR/TCR receptor repertoire;    EBV lymphocytes;    Cancer cell lines;   

DOI  :  10.1186/s12885-018-4840-5

来源: DOAJ

【 摘 要 】

Abstract Background Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. Methods We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. Results Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution. Conclusions Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines.

【 授权许可】

Unknown