Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral Preparations

Frontiers in Cell and Developmental Biology

Tao Pan¹  Christopher Katanski²  Mateusz Halucha²  Christopher Watkins²  Wen Zhang²  Hala Alshammary³  Noah Peña⁴  Matthew M. Hernandez⁵  Viviana Simon⁶  Adolfo Garcia-Sastre⁶  Randy A. Albrecht⁷  Wen-Chun Liu⁷ 

[1] Committee on Microbiology, University of Chicago, Chicago, IL, United States;Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, United States;Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, United States;Department of Pathology, Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States;The Global Health and Emerging Pathogen Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States;

关键词: SARS-CoV-2;    tRNA;    modification;    SRP RNA;    packaging;   

DOI  :  10.3389/fcell.2022.768356

来源: DOAJ

【 摘 要 】

Viruses package host RNAs in their virions which are associated with a range of functions in the viral life cycle. Previous transcriptomic profiling of host RNA packaging mostly focused on retroviruses. Which host RNAs are packaged in other viruses at the transcriptome level has not been thoroughly examined. Here we perform proof-of-concept studies using both small RNA and large RNA sequencing of six different SARS-CoV-2 viral isolates grown on VeroE6 cells to profile host RNAs present in cell free viral preparations and to explore SARS-CoV-2 genomic RNA modifications. We find selective enrichment of specific host transfer RNAs (tRNAs), tRNA fragments and signal recognition particle (SRP) RNA in SARS-CoV-2 viral preparations. Different viral preparations contain the same set of host RNAs, suggesting a common mechanism of packaging. We estimate that a single SARS-CoV-2 particle likely contains up to one SRP RNA and four tRNA molecules. We identify tRNA modification differences between the tRNAs present in viral preparations and those in the uninfected VeroE6 host cells. Furthermore, we find uncharacterized candidate modifications in the SARS-CoV-2 genomic RNA. Our results reveal an under-studied aspect of viral-host interactions that may be explored for viral therapeutics.

【 授权许可】

Unknown