An archaeal protein homologous to mammalian SRP54 and bacterial Ffh recognizes a highly conserved region of SRP RNA

FEBS Letters

Aimi, Tadanori¹  Maeshima, Hideki¹  Okuno, Emi¹  Itoh, Takuzi¹  Morinaga, Tsutomu¹ 

[1] School of Bioresources, Hiroshima Prefectural University, Shobara City, Hiroshima 727-0023, Japan

关键词: Signal recognition particle;    7S RNA;    54 kDa protein of signal recognition particle;    RNA binding protein;    Pyrococcus furiosus;    SRP;    signal recognition particle;    SRP54;    54 kDa protein of signal recognition particle;    SRP19;    19 kDa protein of signal recognition particle;    SRP RNA;    signal recognition particle RNA;    pf-SRP RNA;    Pyrococcus furiosus signal recognition particle RNA;    pf-SRP54;    Pyrococcus furiosus SRP54;    pf-SRP54G;    GTP binding domain in pf-SRP54;    pf-SRP54M;    methionine-rich domain in pf-SRP54;    c. pf-SRP RNA;    conserved region of pf-SRP RNA;    nc. pf-SRP RNA;    non-conserved region of pf-SRP RNA;   

DOI  :  10.1016/S0014-5793(01)02996-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding the 54 kDa protein of signal recognition particle (SRP54) in the hyperthermophilic archaeon Pyrococcus furiosus has been cloned and sequenced. Recombinant P. furiosus SRP54 (pf-SRP54) and the N-terminal G-domain and C-terminal M-domain (pf-SRP54M) of pf-SRP54 with an amino-terminal addition of six histidine residues were expressed in Escherichia coli and subjected to binding experiments for SRP RNA, non-conserved 213-nucleotide RNA (helices 1, 2, 3, 4 and 5) and conserved 107-nucleotide RNA (helices 6 and 8) from SRP RNA. The RNA binding properties of the purified protein were determined by filter binding assays. The histidine-tagged pf-SRP54M bound specifically to the conserved 107-nucleotide RNA in the absence of pf-SRP19, unlike the eukaryotic homologue, with an apparent binding constant (K) of 18 nM.

【 授权许可】

Unknown