Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Biosensors

Zhao Zhang¹  Tiancong Lan¹  Zhen Zhu¹  Ke Liu¹  Zixin Wang²  Xiaowen Huang³ 

[1] Key Laboratory of MEMS of Ministry of Education, Southeast University, Sipailou 2, Nanjing 210018, China;School of Electronics and Information Technology, Sun Yat-Sen University, Xingang Xi Road 135, Guangzhou 510275, China;The First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital), Department of Orthopedics, Nanjing 210029, China;

关键词: electrical impedance spectroscopy;    impedance flow cytometry;    single cell analysis;    microfluidics;   

DOI  :  10.3390/bios11110470

来源: DOAJ

【 摘 要 】

Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.

【 授权许可】

Unknown