Targeted delivery of fat extract by platelet membrane-cloaked nanocarriers for the treatment of ischemic stroke

Journal of Nanobiotechnology

Li Li¹  Qingqing Yin¹  Dongyan Tian¹  Xuewei Yang¹  Deli Zhuge¹  Yijie Chen¹  Wenjie Zhang²  Mingwu Deng²  Guo-Yuan Yang³  Tongtong Xu³  Lin Qi³  Yiyan Guo³  Cheng Wang³  Yixu Jiang³  Zhijun Zhang³  Yongting Wang³  Yaohui Tang³ 

[1] Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University;Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University;School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University;

关键词: Angiogenesis;    Fat extract;    Ischemic stroke;    Platelet membrane;    Neurogenesis;   

DOI  :  10.1186/s12951-022-01461-2

来源: DOAJ

【 摘 要 】

Abstract Background Our previous studies suggest that human fat extract (FE) contains a variety of angiogenic factors and may provide an alternative treatment option for stroke. However, the therapeutic effect is largely limited due to its short half-life, and inaccurate targeting. Results Herein, we leverage the targeting abilities of platelets (PLTs) to the lesion area of stroke and Arg-Gly-Asp (RGD) peptides to the angiogenic blood vessels to develop a biomimetic nanocarrier that capable of delivering FE precisely to treat stroke. The biomimetic nanocarriers are comprised of FE-encapsulated PLGA (poly(lactic-co-glycolic acid)) core enclosed by RGD peptides decorated plasma membrane of PLTs, namely RGD-PLT@PLGA-FE. We found that RGD-PLT@PLGA-FE not only targeted damaged and inflamed blood vessels but also achieved rapid accumulation in the lesion area of ischemic brain. In addition, RGD-PLT@PLGA-FE kept a sustained release behavior of FE at the lesion site, effectively increased its half-life and promoted angiogenesis and neurogenesis with delivering neurotrophic factors including BDNF, GDNF and bFGF to the brain, that ultimately resulted in blood flow increase and neurobehavioral recovery. Conclusions In conclusion, our study provides a new strategy to design a biomimetic system for FE delivery and it is a promising modality for stroke therapy. Graphical Abstract

【 授权许可】

Unknown