Gold Nanobiosensor Based on the Localized Surface Plasmon Resonance is Able to Diagnose Human Brucellosis, Introducing a Rapid and Affordable Method

Nanoscale Research Letters

Mohammad Samare-Najaf¹  Saam Noroozi²  Ali Samareh³  Saeed Taghizadeh⁴  Ahmad Movahedpour⁴  Hassan Askari⁵  Mahdiyar Iravani Saadi⁶  Sina Vakili⁷  Marzieh Alizadeh⁸  Reza Tabrizi⁹  Amir Tajbakhsh^1,10  Amirreza Dehghanian^1,11 

[1] Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences;Department of Biochemistry, Fasa University of Medical Sciences;Department of Clinical Biochemistry, School of Medicine, Kerman University of Medical Sciences;Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences;Gastroenterohepatology Research Center, Shiraz University of Medical Sciences;Hematology Research Center, Shiraz University of Medical Sciences;Infertility Research Center, Shiraz University of Medical Sciences;Laboratory of Basic Sciences, Mohammad Rasul Allah Research Tower, Shiraz University of Medical Sciences;Noncommunicable Diseases Research Center, Fasa University of Medical Sciences;Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences;Trauma Research Center, Shiraz University of Medical Sciences;

关键词: Brucellosis;    Diagnosis;    LSPR;    Nanobiosensor;   

DOI  :  10.1186/s11671-021-03600-4

来源: DOAJ

【 摘 要 】

Abstract Brucellosis is considered as the most common bacterial zoonosis in the world. Although the laboratory findings are the most reliable diagnosis today, the current laboratory methods have many limitations. This research aimed to design and evaluate the performance of a novel technique based on the localized surface plasmon resonance (LSPR) to eliminate or reduce existing shortcomings. For this purpose, smooth lipopolysaccharides were extracted from Brucella melitensis and Brucella abortus and fixed on the surface of the gold nanoparticles through covalent interactions. After some optimizing processes, dynamic light scattering was used to characterize the probe. The detection of captured anti-Brucella antibody was performed by measuring the redshift on LSPR peak followed by the determination of cutoff value, which indicated a significant difference between controls and true positive patients (P value < 0.01). Furthermore, 40 sera from true negative samples and positive patients were used to evaluate the performance of this method by comparing its outcomes with the gold standard (culture), standard tube agglutination test, and anti-brucellosis IgM and IgG levels (ELISA). The sensitivity, specificity, positive predictive value, and negative predictive value showed an appropriate performance of the LSPR-based method (85%, 100%, 100%, and 86%, respectively). The current research results provide a promising fast, convenient, and inexpensive method for detecting the anti-Brucella antibodies in human sera, which can be widely used in medical laboratories to diagnose brucellosis quickly and effectively.

【 授权许可】

Unknown