Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

STAR Protocols

Carmen Mota Reyes¹  Güralp O. Ceyhan¹  Enkhtsetseg Munkhbaatar¹  Linhan Ye²  Alessandra Moretti³  Helmut Friess³  Ümmügülsüm Yurteri³  Ihsan Ekin Demir³  Rouzanna Istvanffy⁴  Fangfang Zhang⁴  H. Erdinc Besikcioglu⁴  Candan Özoğul⁴ 

[1] CRC 1321 Modelling and Targeting Pancreatic Cancer, Munich, Germany;Department of Histology and Embryology, Institution of Health Sciences, Gazi University, Ankara, Turkey;German Cancer Consortium (DKTK), Partner Site Munich, Munich, Germany;Department of Surgery, Klinikum rechts der Isar, Technical University of Munich, School of Medicine, Munich, Germany;

关键词: Cell culture;    Cell isolation;    Cancer;    Model Organisms;    Neuroscience;    Stem Cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons.For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).

【 授权许可】

Unknown