Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus

Genes

Robert E. Parker¹  Shannon D. Manning¹  Rim Al Safadi¹  David Knupp¹  Agnѐs Rosenau² 

[1] Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA;Infectiologie et Santé Publique ISP, Institut National de la Recherche Agronomique, Université de Tours, Equipe Bactéries et Risque Materno-fœtal, UMR1282 Tours, France;

关键词: Streptococcus agalactiae;    quorum sensing;    colonization;    group B Streptococcus;   

DOI  :  10.3390/genes8010023

来源: DOAJ

【 摘 要 】

Streptococcus agalactiae (group B Streptococcus; GBS) is a common inhabitant of the genitourinary and/or gastrointestinal tract in up to 40% of healthy adults; however, this opportunistic pathogen is able to breach restrictive host barriers to cause disease and persist in harsh and changing conditions. This study sought to identify a role for quorum sensing, a form of cell to cell communication, in the regulation of the fibrinogen-binding (rgfBDAC) two-component system and the ability to associate with decidualized endometrial cells in vitro. To do this, we created a deletion in rgfD, which encodes the putative autoinducing peptide, in a GBS strain belonging to multilocus sequence type (ST)-17 and made comparisons to the wild type. Sequence variation in the rgf operon was detected in 40 clinical strains and a non-synonymous single nucleotide polymorphism was detected in rgfD in all of the ST-17 genomes that resulted in a truncation. Using qPCR, expression of rgf operon genes was significantly decreased in the ST-17 ΔrgfD mutant during exponential growth with the biggest difference (3.3-fold) occurring at higher cell densities. Association with decidualized endometrial cells was decreased 1.3-fold in the mutant relative to the wild type and rgfC expression was reduced 22-fold in ΔrgfD following exposure to the endometrial cells. Collectively, these data suggest that this putative quorum sensing molecule is important for attachment to human tissues and demonstrate a role for RgfD in GBS pathogenesis through regulation of rgfC.

【 授权许可】

Unknown