Determination of hemolytic Cytotoxicity and Antibacterial Activity of Conocarpus Lancifolius Aqueous leaves Extract

2nd International Scientific Conference (Pure Sciences, Brilliant Creativity and Renewed Building)

Mohammed, Saddeq A.^1 ; Mousa, Hassan M.^2 ; Alwan, Abdoun H.^1

University of Kerbala, College of Science, Department of Biology, Iraq^1

University of Kerbala, Iraq Collage of Pharmacy, Iraq^2

关键词: Anti-bacterial activity;    Conocarpus lancifolius;    Diffusion method;    MIC and MBC;    Minimum bactericidal concentration (MBC);    Minimum inhibitory concentration;    Streptococcus agalactiae;    Yersinia enterocolitica;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/571/1/012045/pdf
DOI  :  10.1088/1757-899X/571/1/012045

来源: IOP

【 摘 要 】

A total of 32 samples of Conocarpus lancifolius leaves were collected from different regions of holy Kerbala city of Iraq. All samples were undergone to cold aqueous extraction by sonication method. Subsequently, the extraction was tested against six pathogenic bacteria which they were Acintobacter baumanni, Proteus mirabilis, Yersinia enterocolitica, Enterococcus faecalis, Staphylococcus aureus and Streptococcus agalactiae. So, antibacterial activity were determined by well diffusion method as well as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined to each Bacterium. Also, the concentrations of the extraction that used in well diffusion method were 50, 100, 150 and 200 μg/ml. However, all concentrations showed significant differences with significant increase between each other at probability >0.05 and >0.01 for For each concentration. Moreover, all bacteria showed biggest inhibition zone at conc. of 200 μg/ml. Also, the biggest inhibition zone was 22.5 mm for Yersinia enterocolitica, while at the same concentration Acintobacter baumanni, Proteus mirabilis, Enterococcus faecalis, Staphylococcus aureus and Streptococcus agalactiae showed 21.3 μg/ml, 22.0 μg/ml, 15.3 μg/ml, 19.0 μg/ml and 16.2g/ml respectively. Whereas, lowest MIC was 2 μg/ml for Staphylococcus aureus and MBC was 8μg/ml for the same bacteria. Whereas, the MIC of Acinetobacter baumanni, Enterococcus faecalis, Streptococcus agalctiae, Proteus mirabilis and Yersinia enterocolitica was 20, 9, 7, 9 and 8 μg/ml respectively, while the minimum bactericidal concentration (MBC) for these bacteria was 36, 16, 14, 15 and 14 μg/ml respectively. On the other hand hemolytic cytotoxicity of aqueous leaf extract of C. lancifolius showed 1.72% of erythrocyte hemolysis for healthy non-smoker human.