Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

STAR Protocols

Jill Hermans¹  Frédéric M. Vaz¹  Yorrick R.J. Jaspers²  Bauke V. Schomakers³  Gajja Salomons³  Riekelt H. Houtkooper³  Michel van Weeghel³ 

[1] Core Facility Metabolomics, Amsterdam UMC, Location AMC, Meibergdreef 9, AZ 1105 Amsterdam, the Netherlands;Corresponding author;Laboratory Genetic Metabolic Diseases, Amsterdam UMC, Location AMC, Meibergdreef 9, AZ 1105 Amsterdam, the Netherlands;

关键词: Health Sciences;    Mass Spectrometry;    Metabolism;    Metabolomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process.For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

【 授权许可】

Unknown