A Novel Seed-Dressing Formulation Based on an Improved Mutant Strain of Trichoderma virens, and Its Field Evaluation

Frontiers in Microbiology

Somnath Bhattacharyya¹  Rajib Nath¹  Ananya Ghosh¹  Anand K. Tewari²  A. S. Kotasthane³  N. Khare³  Parshuram Rathod³  Kishan Kumar Sharma³  R. K. S. Tiwari³  A. R. Wasnikar⁴  Meenakshi Arya⁵  Sayaji T. Mehetre⁶  Gopi Muthukathan⁶  Prasun K. Mukherjee⁶  P. D. Sherkhane⁶  D. R. Saxena⁷  D. Pathak⁸ 

[1] Department of Agronomy, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, India;Department of Plant Pathology, G. B. Pant University of Agriculture and Technology, Pantnagar, India;Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India;Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, India;Department of Plant Pathology, Rani Lakshmi Bai Central Agricultural University, Jhansi, India;Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India;R.A.K. College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Sehore, India;Regional Agricultural Research Station, Assam Agricultural University, Shillongani, India;

关键词: Trichoderma;    mutant;    tamarind seeds;    formulation;    chickpea;    lentil;   

DOI  :  10.3389/fmicb.2019.01910

来源: DOAJ

【 摘 要 】

Using gamma-ray-induced mutagenesis, we have developed a mutant (named G2) of Trichoderma virens that produced two- to three-fold excesses of secondary metabolites, including viridin, viridiol, and some yet-to-be identified compounds. Consequently, this mutant had improved antibiosis against the oomycete test pathogen Pythium aphanidermatum. A transcriptome analysis of the mutant vis-à-vis the wild-type strain showed upregulation of several secondary-metabolism-related genes. In addition, many genes predicted to be involved in mycoparasitism and plant interactions were also upregulated. We used tamarind seeds as a mass multiplication medium in solid-state fermentation and, using talcum powder as a carrier, developed a novel seed dressing formulation. A comparative evaluation of the wild type and the mutant in greenhouse under high disease pressure (using the test pathogen Sclerotium rolfsii) revealed superiority of the mutant over wild type in protecting chickpea (Cicer arietinum) seeds and seedlings from infection. We then undertook extensive field evaluation (replicated micro-plot trials, on-farm demonstration trials, and large-scale trials in farmers’ fields) of our mutant-based formulation (named TrichoBARC) for management of collar rot (S. rolfsii) in chickpea and lentil (Lens culinaris) over multiple locations in India. In certain experiments, other available formulations were included for comparison. This formulation consistently, over multiple locations and years, improved seed germination, reduced seedling mortality, and improved plant growth and yield. We also noticed growth promotion, improved pod bearing, and early flowering (7–10 days) in TrichoBARC-treated chickpea and lentil plants under field conditions. In toxicological studies in animal models, this formulation exhibited no toxicity to mammals, birds, or fish.

【 授权许可】

Unknown