Transcriptome Sequencing of the Pale Anemones (Exaiptasia diaphana) Revealed Functional Peptide Gene Resources of Sea Anemone

Frontiers in Marine Science

Aihua Jin¹  Tianle Tang²  Yanling Liao²  Jinxing Fu²  Bingmiao Gao²  Yanbin He³  Qiong Shi³  Chao Peng³ 

[1] Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD, Australia;Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Pharmacy, Hainan Medical University, Haikou, China;Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, Shenzhen Huahong Marine Biomedicine Co. Ltd., BGI Marine, BGI, Shenzhen, China;

关键词: sea anemone;    Exaiptasia diaphana;    transcriptomics;    cnidarian;    toxins;    insulin-like peptide;   

DOI  :  10.3389/fmars.2022.856501

来源: DOAJ

【 摘 要 】

Sea anemone venom is a marine drug resource library with pharmacological and biotechnology value, and it contains complex and diverse functional peptide neurotoxins. However, the venom components of only a limited number of sea anemone species have been globally evaluated by transcriptomics and proteomics. In this study, 533 putative protein as well as peptide toxin sequences were found on a large scale from dissimilar developmental stages of sea anemone Exaiptasia diaphana, which can be divided into 75 known superfamilies according to the predicted functions. Among them, the proportion of protein is 72.98%, and its main families are metalloproteases, chymotrypsinogen like, collagen, pancreatic lipase-associated protein like, and G-protein coupled receptor, while the proportion of peptides is 27.02%, and main families are ShK domain, thrombin, Kunitz-type, defensin, as well as insulin-like peptide. Finally, typical anemone peptide neurotoxins were screened, and the 3D structure and pharmacological activity of these anemone peptide neurotoxins were predicted by homology modeling. We elucidate on a valuable high-throughput approach for obtaining sea anemone proteins and peptides. Our findings form the basis for targeted studies on the diversity as well as pharmacological effects of sea anemone peptide neurotoxins.

【 授权许可】

Unknown