Imaging of hypoxia in small animals with 18F fluoromisonidasole

Nukleonika

Drzał Agnieszka¹  Elas Martyna¹  Cheda Łukasz²  Rogulski Zbigniew²  Gerszewska Marina³  Cudny Michał³  Kilian Krzysztof⁴ 

[1] Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 30-387 Krakow, Poland;Faculty of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland;Faculty of Physics, University of Warsaw, 5 Pasteura Str., 02-093 Warsaw, Poland;Heavy Ion Laboratory, University of Warsaw, 5A Pasteura Str., 02-093 Warsaw, Poland;

关键词: 18F-fluoromisonidazole;    radiopharmaceuticals;    hypoxia imaging;   

DOI  :  10.1515/nuka-2016-0037

来源: DOAJ

【 摘 要 】

A method of automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) for application in preclinical studies on small animals was presented. A remote-controlled synthesizer Synthra RNplus was used for nucleophilic substitution of NITTP (1′-(2′-nitro-1-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonyl-propanediol) with 18F anion. Labeling of 5 mg of precursor was performed in anhydrous acetonitrile at 100°C for 10 min, and the hydrolysis with HCl was performed at 100°C for 5 min. Final purification was done with high-performance liquid chromatography (HPLC) and the radiochemical purity of radiotracer was higher than 99%. Proposed [18F]FMISO synthesis was used as a reliable tool in studies on hypoxia in Lewis lung carcinoma (LLC) in mouse models.

【 授权许可】

Unknown