Immunohistochemical investigations on Brucella ceti-infected, neurobrucellosis-affected striped dolphins (Stenella coeruleoalba)

Veterinaria Italiana

Clotilde Angelucci¹  Giovanni Di Guardo¹  Marina Baffoni¹  Roberto Giacominelli Stuffler¹  Eva Sierra²  Antonio Fernández²  Giuseppe Lucifora³  Carla Grattarola⁴  Barbara Iulini⁴  Antonio Petrella⁵  Gabriella Di Francesco⁶  Manuela Tittarelli⁷  Mirella Luciani⁷  Tiziana Di Febo⁷  Enzo Ruggieri⁷  Ludovica Di Renzo⁷  Anna Rita D'Angelo⁷  Antonio Petrini⁷  Osvaldo Matteucci⁷ 

[1] Faculty of Veterinary Medicine, Località Piano d'Accio, University of Teramo, Italy;Institute of Animal Health, University of Las Palmas de Gran Canaria, Veterinary School, C/Transmontaña s/n, 35416, Arucas, Las Palmas;Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici (NA), Italy;Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy;Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy.;Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’, Campo Boario, 64100 Teramo, Italy. Tel.: + 39 0861 332420, e-mail: [e-mail];Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’, Campo Boario, 64100 Teramo, Italy;

关键词: Brucella ceti;    Immunohistochemistry;    Neurobrucellosis;    Stenella coeruleoalba;   

DOI  :  10.12834/VetIt.1920.10224.2

来源: DOAJ

【 摘 要 】

Bacteria of the genus Brucella cause brucellosis, an infectious disease common to humans as well as to terrestrial and aquatic mammals. Since 1994 several cases of Brucella spp. infection have been reported in marine mammals worldwide. While sero-epidemiological data suggest that Brucella spp. infection is widespread globally, detecting Brucella spp.-associated antigens by immunohistochemistry (IHC) in tissues from infected animals is often troublesome. The present study was aimed at investigating, by means of IHC based upon the utilization of an anti-Brucella LPS monoclonal antibody (MAb), the central nervous system (CNS) immunoreactivity shown by B. ceti-infected, neurobrucellosis-affected striped dolphins. The aforementioned MAb, previously characterized by means of ELISA and Western Blotting techniques, was able to immunohistochemically detect smooth brucellae both within the CNS from B. ceti-infected striped dolphins and within a range of tissues from Brucella spp.-infected domestic ruminants. In conclusion, the results of the present study are of relevance both from the B. ceti infection's diagnostic and pathogenetic standpoints.

【 授权许可】

Unknown