Caenorhabditis elegans as a Model Host to Monitor the Candida Infection Processes

Journal of Fungi

Luca Issi¹  AsmaaB. Elkabti¹  ReetaP. Rao¹ 

[1] Worcester Polytechnic Institute, Worcester, MA 01609, USA;

关键词: Candida albicans;    virulence factors;    innate immunity;    infectious disease;    host-pathogen interactions;    model host;   

DOI  :  10.3390/jof4040123

来源: DOAJ

【 摘 要 】

C. elegans has several advantages as an experimental host for the study of infectious diseases. Worms are easily maintained and propagated on bacterial lawns. The worms can be frozen for long term storage and still maintain viability years later. Their short generation time and large brood size of thousands of worms grown on a single petri dish, makes it relatively easy to maintain at a low cost. The typical wild type adult worm grows to approximately 1.5 mm in length and are transparent, allowing for the identification of several internal organs using an affordable dissecting microscope. A large collection of loss of function mutant strains are readily available from the C. elegans genetic stock center, making targeted genetic studies in the nematode possible. Here we describe ways in which this facile model host has been used to study Candida albicans, an opportunistic fungal pathogen that poses a serious public health threat.

【 授权许可】

Unknown