Tasco®: A Product of Ascophyllum nodosum Enhances Immune Response of Caenorhabditis elegans Against Pseudomonas aeruginosa Infection

Marine Drugs

Saveetha Kandasamy²  Wajahatullah Khan³  Franklin Evans¹  Alan T. Critchley¹ 

[1] Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS, B3B 1X8, Canada;Department of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro, NS, B2B 5E3, Canada;Genome Research Chair Unit, Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;

关键词: Tasco^®;    Ascophyllum nodosum;    Caenorhabiditis elegans;    Pseudomonas aeruginosa;    virulence factors;    innate immunity;    gene expression;    quorum sensing;   

DOI  :  10.3390/md10010084

来源: mdpi

【 摘 要 】

The effects of Tasco^®, a product made from the brown seaweed (Ascophyllum nodosum) were tested for the ability to protect Caenorhabditis elegans against Pseudomonas aeruginosa infection. A water extract of Tasco^® (TWE) reduced P. aeruginosa inflicted mortality in the nematode. The TWE, at a concentration of 300 µg/mL, offered the maximum protection and induced the expression of innate immune response genes viz.; zk6.7 (Lypases), lys-1 (Lysozyme), spp-1 (Saponin like protein), f28d1.3 (Thaumatin like protein), t20g5.7 (Matridin SK domain protein), abf-1 (Antibacterial protein) and f38a1.5 (Lectin family protein). Further, TWE treatment also affected a number of virulence components of the P. aeuroginosa and reduced its secreted virulence factors such as lipase, proteases and toxic metabolites; hydrogen cyanide and pyocyanin. Decreased virulence factors were associated with a significant reduction in expression of regulatory genes involved in quorum sensing, lasI, lasR, rhlI and rhlR. In conclusion, the TWE-treatment protected the C. elegans against P. aeruginosa infection by a combination of effects on the innate immunity of the worms and direct effects on the bacterial quorum sensing and virulence factors.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland