Interactive 360° video to “Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis”

Electronic Journal of Biotechnology

Andrea Müller-Pavez¹  Mauricio Vergara-Castro¹  Eduardo Caballero-Valdés²  Carmen Soto-Maldonado³  Claudia Altamirano³  María Elvira Zúñiga-Hansen⁴  John Jara-Quezada⁴ 

[1] Corresponding authors.;Instituto de Química, Pontificia Universidad Católica de Valparaíso, Av. Universidad 330, Curauma-Placilla, Valparaíso, Chile;Centro Regional de Estudios en Alimentos Saludables, GORE-CONICYT R17A10001, Av. Universidad 330, Curauma-Placilla, Valparaíso, Chile;Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil, 2085 Valparaíso, Chile;

关键词: 360°;    360° video;    Antiproliferative;    Apoptosis;    Green husk;    HL-60 cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Juglone is a naphthoquinone currently obtained by chemical synthesis with biological activities including antitumor activity. Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines. Results: Walnut green husk ethanolic extract was obtained as 169.1 mg juglone/100 g Green Husk and antioxidant activity (ORAC) of 44,920 μmol Trolox Equivalent/100 g DW Green Husk. At 1 μM juglone in HL-60 cell culture, green husk extract showed an antiproliferative effect, but pure juglone did not; under these conditions, normal fibroblast cells were not affected. A dose-dependent effect on mitochondrial membrane potential loss was observed. Apoptosis of HL-60 was detected at 10 μM juglone. Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions. Conclusions: Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells. How to cite: Soto-Maldonado C, Vergara-Castro M, Jara-Quezada J, et al. Interactive 360° video to: Polyphenolic extracts of walnut (Juglans regia) green husk containing juglone inhibit the growth of HL-60 cells and induce apoptosis. Electron J Biotechnol 2022;57. https://doi.org/10.1016/j.ejbt.2022.04.002.

【 授权许可】

Unknown