Transcriptomic analysis of synchrony and productivity in self-cycling fermentation of engineered yeast producing shikimic acid

Biotechnology Reports

Lisa Y. Stein¹  Roman Vincent C. Agustin²  Yusheng Tan²  Dominic Sauvageau² 

[1] Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada;Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada;

关键词: Self-cycling fermentation (SCF);    Saccharomyces cerevisiae;    Transcriptomics;    Shikimic acid;    Synchrony;   

DOI  :  

来源: DOAJ

【 摘 要 】

Industrial fermentation provides a wide variety of bioproducts, such as food, biofuels and pharmaceuticals. Self-cycling fermentation (SCF), an advanced automated semi-continuous fermentation approach, has shown significant advantages over batch reactors (BR); including cell synchrony and improved production. Here, Saccharomyces cerevisiae engineered to overproduce shikimic acid was grown under SCF operation. This led to four-fold increases in product yield and volumetric productivity compared to BR. Transcriptomic analyses were performed to understand the cellular mechanisms leading to these increases. Results indicate an up-regulation of a large number of genes related to the cell cycle and DNA replication in the early stages of SCF cycles, inferring substantial synchronization. Moreover, numerous genes related to gluconeogenesis, the citrate cycle and oxidative phosphorylation were significantly up-regulated in the late stages of SCF cycles, consistent with significant increases in shikimic acid yield and productivity.

【 授权许可】

Unknown