Transcriptomics in human blood incubation reveals the importance of oxidative stress response in Saccharomyces cerevisiae clinical strains

BMC Genomics

Research Article

Lene Jespersen¹  Antje Heyken²  Bernhard Hube³  Silvia Llopis⁴  M Teresa Fernández-Espinar⁴  Roberto Pérez-Torrado⁴  Amparo Querol⁴ 

[1] Department of Food Science, Food Microbiology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark;Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institute (HKI), Jena, Germany;Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institute (HKI), Jena, Germany;Friedrich Schiller University, Jena, Germany;Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, P.O. Box, 73, E-46100, Burjassot, Spain;

关键词: Saccharomyces cerevisiae;    Clinical strains;    Transcriptomics;    Blood;    Oxidative stress;   

DOI  :  10.1186/1471-2164-13-419

 received in 2012-04-04, accepted in 2012-08-20,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundIn recent years an increasing number of yeast infections in humans have been related to certain clinical isolates of Saccharomyces cerevisiae. Some clinical strains showed in vivo and in vitro virulence traits and were able to cause death in mice whereas other clinical strains were avirulent.ResultsIn this work, we studied the transcriptional profiles of two S. cerevisiae clinical strains showing virulent traits and two control non-virulent strains during a blood incubation model and detected a specific transcriptional response of clinical strains. This response involves an mRNA levels increase of amino acid biosynthesis genes and especially oxidative stress related genes. We observed that the clinical strains were more resistant to reactive oxygen species in vitro. In addition, blood survival of clinical isolates was high, reaching similar levels to pathogenic Candida albicans strain. Furthermore, a virulent strain mutant in the transcription factor Yap1p, unable to grow in oxidative stress conditions, presented decreased survival levels in human blood compared with the wild type or YAP1 reconstituted strain.ConclusionsOur data suggest that this enhanced oxidative stress response in virulent clinical isolates, presumably induced in response to oxidative burst from host defense cells, is important to increase survival in human blood and can help to infect and even produce death in mice models.

【 授权许可】

Unknown   
© Llopis et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.