Chlorophyll a interference in phycocyanin and allophycocyanin spectrophotometric quantification

Journal of Limnology

Mariano Bresciani¹  Rosaria Lauceri²  Giuseppe Morabito²  Andrea Lami² 

[1] National Research Council of Italy, Institute for Electromagnetic Sensing of the Environment,Optical Sensing Group;National Research Council of Italy, Institute for the Study of Ecosystems;

关键词: Cyanobacteria;    phycocyanin;    allophycocyanin;    chlorophyll a;    spectrophotometric equation.;   

DOI  :  10.4081/jlimnol.2017.1691

来源: DOAJ

【 摘 要 】

The accurate quantification of cyanobacteria phycobiliproteins is an important aspect in various research topics, such as cyanobacteria ecology and physiology studies, and especially to calibrate algorithms used in remote sensing of cyanobacterial blooms. Here we present a spectroscopic approach, exploiting spectrophotometric equations, aimed at improving the phycocyanin and allophycocyanin quantification when chlorophyll a is present in the phycobiliprotein aqueous extract.

【 授权许可】

Unknown