Energy migration in allophycocyanin‐B trimer with a linker polypeptide: Analysis by the principal multi‐component spectral estimation (PMSE) method

FEBS Letters

Yamazaki, Iwao⁴  Matsuura, Katsumi³  Nishimura, Yoshinobu⁴  Shimada, Keizo³  Yamazaki, Tomoko⁴  Mimuro, Mamoru²  Hirano, Masahiko¹ 

[1] Toray Research Center, Kamakura 248, Japan;National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan;Department of Biology, Faculty of Science, Tokyo Metropolitan University, Hachioji 192-03, Japan;Department of Chemical Process Engineering, Faculty of Engineering, Hokkaido University, Sapporo 060, Japan

关键词: Allophycocyanin;    Energy transfer;    Photosynthesis;    Picosecond spectroscopy;    Spectral analysis;    Synechococcus elongatus;    αAPB;    α subunit a allophycocyanin B;    APC;    allophycocyanin;    DAS;    decay-associated spectra;    kDa;    kilodaltons;    PC;    phycocyanin;    PMSE;    principal multi-component spectral estimation;    PMSF;    phenylmethylsulfonylflouride;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(94)01009-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Energy migration processes in allophycocyanin-B trimer with a linker polypeptide were analyzed using the principal multi-component spectral estimation (PMSE) method, which does not require assumption of component number, decay function, or the spectral band shape. We determined the number of spectral components showing independent kinetic behavior by the eigenvalue of an auto-correlation matrix, and further the spectra of the components and their rise and decay curves. Two decay components were resolved at 20°C: One corresponded to the decay of one type of β-84 chromophore, and the other to the decay from the thermally equilibrated state between another type of β-84 chromophore and the α-allophycocyanin B chromophore. An additional slow decay process was resolved at - 196°C. We also compared the component spectra obtained using the PMSE method with the decay-associated spectra obtained using the global analysis.

【 授权许可】

Unknown