Stem Cell-Like Properties of CK2β-down Regulated Mammary Cells

Cancers

Baharia Mograbi¹  Magali Prioux²  Eve Duchemin-Pelletier²  Elodie Spreux²  Megghane Baulard²  Laurent Guyon²  Claude Cochet²  Odile Filhol²  Mithila Burute³  Manuel Théry³ 

[1] Biology Department, Inserm, CNRS, IRCAN, Université Côte d’Azur, F-06000 Nice, France;Chemistry and Biology Department, Université Grenoble Alpes, F-38400 Grenoble, France;Laboratoire de Physiologie Cellulaire et Végétale, Biosciences & Biotechnology Institute of Grenoble, UMR5168, CEA/INRA/CNRS, F-38054 Grenoble, France;

关键词: protein kinase CK2;    stem cell;    breast cancer;    EMT;    epithelial plasticity;   

DOI  :  10.3390/cancers9090114

来源: DOAJ

【 摘 要 】

The ubiquitous protein kinase CK2 has been demonstrated to be overexpressed in a number of human tumours. This enzyme is composed of two catalytic α or α’ subunits and a dimer of β regulatory subunits whose expression levels are probably implicated in CK2 regulation. Several recent papers reported that unbalanced expression of CK2 subunits is sufficient to drive epithelial to mesenchymal transition, a process involved in cancer invasion and metastasis. Herein, through transcriptomic and miRNA analysis together with comparison of cellular properties between wild type and CK2β-knock-down MCF10A cells, we show that down-regulation of CK2β subunit in mammary epithelial cells induces the acquisition of stem cell-like properties associated with perturbed polarity, CD44high/CD24low antigenic phenotype and the ability to grow under anchorage-independent conditions. These data demonstrate that a CK2β level establishes a critical cell fate threshold in the control of epithelial cell plasticity. Thus, this regulatory subunit functions as a nodal protein to maintain an epithelial phenotype and its depletion drives breast cell stemness.

【 授权许可】

Unknown