Construction of a High-Density Genetic Map Based on SLAF Markers and QTL Analysis of Leaf Size in Rice

Frontiers in Plant Science

Linlin Hou¹  YunxiaFang¹  Dawei Xue¹  Qian Qian²  Yi Wen²  Longbiao Guo³  Qiang Zhang³  Hao Wu³  Yueying Wang³  Guangheng Zhang³  Xuemei Deng³  Lixin Zhu³  Li Zhu³  Guang Chen³  Lan Shen³  Dali Zeng³  Peng Hu³  Guojun Dong³  Jiang Hu³  Zhenyu Gao³  Yiqing Tan³  Deyong Ren³ 

[1] College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China;Rice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province, Shenyang, China;State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China;

关键词: rice;    leaf;    quantitative trait loci;    genetic map;    specific length amplified fragment sequencing;    fine mapping;   

DOI  :  10.3389/fpls.2020.01143

来源: DOAJ

【 摘 要 】

Leaf shape is an important agronomic trait for constructing an ideal plant type in rice, and high-density genetic map is facilitative in improving accuracy and efficiency for quantitative trait loci (QTL) analysis of leaf trait. In this study, a high-density genetic map contained 10,760 specific length amplified fragment sequencing (SLAF) markers was established based on 149 recombinant inbred lines (RILs) derived from the cross between Rekuangeng (RKG) and Taizhong1 (TN1), which exhibited 1,613.59 cM map distance with an average interval of 0.17 cM. A total of 24 QTLs were detected and explained the phenotypic variance ranged from 9% to 33.8% related to the leaf morphology across two areas. Among them, one uncloned major QTL qTLLW1 (qTLL1 and qTLLW1) involved in regulating leaf length and leaf width with max 33.8% and 22.5% phenotypic variance respectively was located on chromosome 1, and another major locus qTLW4 affecting leaf width accounted for max 25.3% phenotypic variance was mapped on chromosome 4. Fine mapping and qRT-PCR expression analysis indicated that qTLW4 may be allelic to NAL1 (Narrow leaf 1) gene.

【 授权许可】

Unknown