Discovery of Pyranoviolin A and Its Biosynthetic Gene Cluster in Aspergillus violaceofuscus

Frontiers in Microbiology

Yudai Matsuda¹  Lin Chen²  Xingxing Wei²  Jian-Wei Tang³ 

[1] City University of Hong Kong Shenzhen Research Institute, Shenzhen, China;Department of Chemistry, City University of Hong Kong, Kowloon, Hong Kong, China;Department of Ocean Science and Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China;

关键词: Aspergillus violaceofuscus;    natural products;    biosynthesis;    fungal secondary metabolites;    polyketide-non-ribosomal peptides;   

DOI  :  10.3389/fmicb.2020.562063

来源: DOAJ

【 摘 要 】

A new polyketide-non-ribosomal peptide hybrid molecule, pyranoviolin A (1), was discovered from the genome-sequenced fungus Aspergillus violaceofuscus CBS 115571 and was characterized to be the first pyranonigrin analog harboring the C-3 methoxy group. Examination of the genome sequence of the fungus identified a putative biosynthetic gene cluster of 1, which was designated as the pyv cluster. The gene deletion experiment of the polyketide synthase (PKS)-non-ribosomal peptide synthetase (NRPS) hybrid gene in the cluster confirmed the involvement of the pyv cluster in the pyranoviolin A biosynthesis. Finally, a plausible biosynthetic route leading to 1 has been proposed based on the bioinformatic analysis. Our study indicates that metabolite analysis of genome-sequenced microorganisms whose metabolites have been largely unexplored facilitates the discovery of new secondary metabolites along with their biosynthetic gene clusters.

【 授权许可】

Unknown