An Integration of Genome-Wide Association Study and Gene Co-expression Network Analysis Identifies Candidate Genes of Stem Lodging-Related Traits in Brassica napus

Frontiers in Plant Science

Hao Li¹  Biyun Chen¹  Kun Xu¹  Qian Huang¹  Lixia Li¹  Fugui Zhang¹  Guixin Yan¹  Xiaoming Wu¹  Xi Cheng¹  Guizhen Gao¹  Liping Zhang¹  Jihong Hu¹  Zaiyun Li²  Hongge Li² 

[1] Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Wuhan, China;National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, National Center of Oil Crop Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China;

关键词: Brassica napus L.;    stem lodging-related traits;    genome-wide association study;    gene co-expression network;    candidate genes;   

DOI  :  10.3389/fpls.2018.00796

来源: DOAJ

【 摘 要 】

Lodging is a persistent problem which severely reduce yield and impair seed quality in rapeseed (Brassica napus L.). Enhancing stem strength (SS) has proven to be an effective approach to decrease lodging risk. In the present study, four interrelated stem lodging-related traits, including stem breaking resistance (SBR), stem diameter (SD), SS, and lodging coefficient (LC), were investigated among 472 rapeseed accessions. A genome-wide association study (GWAS) using Brassica 60K SNP array for stem lodging-related traits identified 67 significantly associated quantitative trait loci (QTLs) and 71 candidate genes. In parallel, a gene co-expression network based on transcriptome sequencing was constructed. The module associated with cellulose biosynthesis was highlighted. By integrating GWAS and gene co-expression network analysis, some promising candidate genes, such as ESKIMO1 (ESK1, BnaC08g26920D), CELLULOSE SYNTHASE 6 (CESA6, BnaA09g06990D), and FRAGILE FIBER 8 (FRA8, BnaC04g39510D), were prioritized for further research. These findings revealed the genetic basis underlying stem lodging and provided worthwhile QTLs and genes information for genetic improvement of stem lodging resistance in B. napus.

【 授权许可】

Unknown