Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model

Frontiers in Bioengineering and Biotechnology

Joshua R. Mauney¹  Maryrose P. Sullivan³  Antoine E. Khoury⁴  Sarah A. Holzman⁴  Zhamshid Okhunov⁵  Gokhan Gundogdu⁵  Hazem Orabi⁵  Faith Veneri⁵  Stephanie Starek⁵ 

[1] Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, United States;Department of Surgery and Harvard Medical School, Boston, MA, United States;Department of Surgery, Brigham and Women’s Hospital, Boston, MA, United States;Department of Urology, Children’s Hospital of Orange County (CHOC), Orange, CA, United States;Department of Urology, University of California, Irvine, Irvine, CA, United States;Division of Urology, Veterans Affairs Boston Healthcare System, West Roxbury, MA, United States;

关键词: biomaterials;    silk fibroin;    corporoplasty;    tissue engineering;    regeneration;   

DOI  :  10.3389/fbioe.2021.791119

来源: DOAJ

【 摘 要 】

The use of autologous tissue grafts for tunica albuginea repair in Peyronie’s disease and congenital chordee is often restricted by limited tissue availability and donor site morbidity, therefore new biomaterial options are needed. In this study, bi-layer silk fibroin (BLSF) scaffolds were investigated to support functional tissue regeneration of tunica albuginea in a rabbit corporoplasty model. Eighteen adult male, New Zealand white rabbits were randomized to nonsurgical controls (NSC, N = 3), or subjected to corporoplasty with BLSF grafts (N = 5); decellularized small intestinal submucosa (SIS) matrices (N = 5); or autologous tunica vaginalis (TV) flaps (N = 5). End-point evaluations were cavernosography, cavernosometry, histological, immunohistochemical, and histomorphometric assessments. Maximum intracorporal pressures (ICP) following papaverine-induced erection were similar between all groups. Eighty percent of rabbits repaired with BLSF scaffolds or TV flaps achieved full rigid erections, compared to 40% of SIS reconstructed animals. Five-minute peak erections were maintained in 60% of BLSF rabbits, compared to 20% of SIS and TV flap reconstructed rabbits. Graft perforation occurred in 60% of TV group at maximum ICP compared to 20% of BLSF cohort. Neotissues supported by SIS and BLSF scaffolds were composed of collagen type I and elastin fibers similar to NSC. SIS and TV flaps showed significantly elevated levels of corporal fibrosis relative to NSC with a corresponding decrease in corporal smooth muscle cells expressing contractile proteins. BLSF biomaterials represent emerging platforms for corporoplasty and produce superior functional and histological outcomes in comparison to TV flaps and SIS matrices for tunica albuginea repair.

【 授权许可】

Unknown