Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering

International Journal of Molecular Sciences

卷:22

AngelaR. Armiento¹  Peter Behrendt²  Jan-Tobias Weitkamp³  Andreas Bayer³  Bastian Nußpickel³  Philipp Arnold³  Ralph Lucius³  Bodo Kurz³  Ralf Smeets⁴  FelixN. Schmidt⁵  Michael Wöltje⁶  Dilbar Aibibu⁶  Chokri Cherif⁶  David Eglin⁷ 

[1] AO Research Institute Davos, 7270 Davos Platz, Switzerland;

[2] Clinic for Orthopedic and Trauma Surgery, University Medical Center Schleswig-Holstein, Campus Kiel, 24015 Kiel, Germany;

[3] Department of Anatomy, Christian-Albrechts-University Kiel, 24118 Kiel, Germany;

[4] Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;

[5] Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, 22529 Hamburg, Germany;

[6] Institute of Textile Machinery and High Performance Material Technology, 01069 Dresden, Germany;

[7] Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, F-42023 Saint-Etienne, France;

关键词: cartilage;    hyaluronic acid;    chondrocytes;    silk fibroin;    autologous chondrocyte implantation;    biomaterials;   

DOI  :  10.3390/ijms22073635

来源: DOAJ

【 摘 要 】

A continuing challenge in cartilage tissue engineering for cartilage regeneration is the creation of a suitable synthetic microenvironment for chondrocytes and tissue regeneration. The aim of this study was to develop a highly tunable hybrid scaffold based on a silk fibroin matrix (SM) and a hyaluronic acid (HA) hydrogel. Human articular chondrocytes were embedded in a porous 3-dimensional SM, before infiltration with tyramine modified HA hydrogel. Scaffolds were cultured in chondropermissive medium with and without TGF-β1. Cell viability and cell distribution were assessed using CellTiter-Blue assay and Live/Dead staining. Chondrogenic marker expression was detected using qPCR. Biosynthesis of matrix compounds was analyzed by dimethylmethylene blue assay and immuno-histology. Differences in biomaterial stiffness and stress relaxation were characterized using a one-step unconfined compression test. Cell morphology was investigated by scanning electron microscopy. Hybrid scaffold revealed superior chondro-inductive and biomechanical properties compared to sole SM. The presence of HA and TGF-β1 increased chondrogenic marker gene expression and matrix deposition. Hybrid scaffolds offer cytocompatible and highly tunable properties as cell-carrier systems, as well as favorable biomechanical properties.

【 授权许可】

Unknown