Potentiation of in vitro apoptotic effects of δ-Tocotrienol and Jerantinine A on human lung adenocarcinoma cells

Journal of HerbMed Pharmacology

Hwei-San Loh¹  Ibrahim Babangida Abubakar¹ 

[1] School of Biosciences, Faculty of Science, The University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor Darul Ehsan, Malaysia;

关键词: δ-Tocotrienol;    Jerantinine A;    Synergism;    Apoptosis;    Microtubules;   

DOI  :  10.15171/jhp.2019.49

来源: DOAJ

【 摘 要 】

Introduction: The apoptotic effects of single-compound and combined sub-effective concentrations of δ-tocotrienol and jerantinine A on human lung adenocarcinoma (A549) cells were investigated. Methods: Assays including cell viability, histochemical and immunofluorescence staining techniques, flow cytometry and enzyme activity were used. Results: The combination of δ-tocotrienol with jerantinine A at sub-effective concentrations induced a synergistic effect and improved selective toxicity towards cancerous A549 cells over normal lung fibroblast (MRC5) cells compared to the single-compound jerantinine. Morphological features of apoptosis were evident on treated A549 cells. Combined sub-effective concentrations of δ-tocotrienol with jerantinine A induced a predominantly G2/M cell cycle arrest and characterised by a disruption of microtubular networks mediated via caspase 8, 9 and 3 enzymatic activities. Conclusion: These findings demonstrated improved potency in vitro and reduced dose-related toxicity of jerantinine A to normal cells through prospective combined treatment between low-concentration δ-tocotrienol and jerantinine A for lung cancer.

【 授权许可】

Unknown