Unraveling Heterogeneity in Transcriptome and Its Regulation Through Single-Cell Multi-Omics Technologies

Frontiers in Genetics

Yih-Cherng Liou¹  Nadia Omega Cipta¹  Yuin-Han Loh²  Kiyofumi Hamashima³  Cheng Gee Koh⁵  Qiao Rui Xing⁵ 

[1] Department of Biological Sciences, National University of Singapore, Singapore, Singapore;Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore;Epigenetics and Cell Fates Laboratory, Institute of Molecular and Cell Biology, A∗STAR, Singapore, Singapore;NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore;School of Biological Sciences, Nanyang Technological University, Singapore, Singapore;

关键词: multimodal single-cell techniques;    spatial transcriptome;    transcriptome;    chromatin accessibility;    integrative analysis;   

DOI  :  10.3389/fgene.2020.00662

来源: DOAJ

【 摘 要 】

Cellular heterogeneity plays a pivotal role in tissue homeostasis and the disease development of multicellular organisms. To deconstruct the heterogeneity, a multitude of single-cell toolkits measuring various cellular contents, including genome, transcriptome, epigenome, and proteome, have been developed. More recently, multi-omics single-cell techniques enable the capture of molecular footprints with a higher resolution by simultaneously profiling various cellular contents within an individual cell. Integrative analysis of multi-omics datasets unravels the relationships between cellular modalities, builds sophisticated regulatory networks, and provides a holistic view of the cell state. In this review, we summarize the major developments in the single-cell field and review the current state-of-the-art single-cell multi-omic techniques and the bioinformatic tools for integrative analysis.

【 授权许可】

Unknown