Supragingival Plaque Microbiome Ecology and Functional Potential in the Context of Health and Disease

mBio

Jeffrey M. Craig¹  Chris L. Dupont²  Josh L. Espinoza²  Andres Gomez³  Claire Kuelbs³  Manolito Torralba³  Jason M. Inman³  Sarah K. Highlander³  Derek M. Harkins⁴  Karen E. Nelson⁴  Marcus B. Jones⁵  Pamela Leong⁶  Richard Saffery⁶  Toby Hughes⁷  Michelle Bockmann⁷ 

[1] Centre for Molecular and Medical Research, School of Medicine, Deakin University, Geelong, VIC, Australia;Department of Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, California, USA;Departments of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, California, USA;Departments of Human Biology and Genomic Medicine, J. Craig Venter Institute, Rockville, Maryland, USA;Human Longevity Institute, La Jolla, California, USA;Murdoch Children’s Research Institute and Department of Pediatrics, University of Melbourne, Royal Children’s Hospital, Parkville, VIC, Australia;School of Dentistry, The University of Adelaide, Adelaide, SA, Australia;

关键词: Streptococcus;    metabolism;    metagenomics;    microbial ecology;    oral microbiology;   

DOI  :  10.1128/mBio.01631-18

来源: DOAJ

【 摘 要 】

ABSTRACT To address the question of how microbial diversity and function in the oral cavities of children relates to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. Caries phenotypes contained statistically significant enrichments in specific genome abundances and distinct community composition profiles, including strain-level changes. Metabolic pathways that are statistically associated with caries include several sugar-associated phosphotransferase systems, antimicrobial resistance, and metal transport. Numerous closely related previously uncharacterized microbes had substantial variation in central metabolism, including the loss of biosynthetic pathways resulting in auxotrophy, changing the ecological role. We also describe the first complete Gracilibacteria genomes from the human microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries. IMPORTANCE Oral health has substantial economic importance, with over $100 billion spent on dental care in the United States annually. The microbiome plays a critical role in oral health, yet remains poorly classified. To address the question of how microbial diversity and function in the oral cavities of children relate to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. This unveiled several new previously uncharacterized but ubiquitous microbial lineages in the oral microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries.

【 授权许可】

Unknown