The expression of vascular endothelial growth factor is affected by hypoxia inducible factor-1α in peritoneum of endometriosis mice treated with genistein

Middle East Fertility Society Journal

Yuyun Yueniwati¹  Sutrisno Sutrisno²  Sylvi Wafda Nur Amellia³ 

[1] Department of Radiology, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia;Division of Fertility, Endocrinology and Reproduction, Department of Obstetric and Ginaecology, Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia;Midwifery Master Study Programme, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia;

关键词: Hypoxia;    Growth factor;    Angiogenesis;    Peritoneal;    Endometriosis;   

DOI  :  10.1016/j.mefs.2015.12.005

来源: DOAJ

【 摘 要 】

This study aimed to investigate whether the genistein is able to decrease the expressions of vascular endothelial growth factor-A (VEGF-A) and hypoxia inducible factor-1α (HIF-1α) in mouse model of endometriosis. Forty female mice (Mus musculus) were divided into eight groups (n = 5 each), including the control (untreated) group, endometriosis group, and endometriosis groups treated with various doses of genistein (50; 100; 200; 300; 400; and 500 mg/day). VEGF-A and HIF-1α analyses were performed by immunohistochemistry. We found significant increases in the VEGF-A and HIF-α expressions in endometriosis group compared to the control group. The increased expressions of VEGF-A and HIF-1α were significantly (p < 0.05) attenuated by the administration of all doses of genistein. In conclusion, in mouse model of endometriosis, genistein potentially inhibits the increase in angiogenesis in peritoneal tissue. Therefore, this result may provide a novel anti-angiogenic treatment strategy for the therapy of endometriosis.

【 授权许可】

Unknown