Nitrogen availability prevents oxidative effects of salinity on wheat growth and photosynthesis by up-regulating the antioxidants and osmolytes metabolism, and secondary metabolite accumulation

BMC Plant Biology

Mohamed El-Esawi¹  Mohamed A. El-Sheikh²  Abdulrahman A. Alatar²  Naheeda Begum³  Qi Maodong³  Cheng Qin³  Xu Xue Dong³  Mohammad Abass Ahanger³  Lixin Zhang³ 

[1] Botany Department, Faculty of Science, Tanta University;Botany and Microbiology Department, College of Science, King Saud University;College of Life Sciences;

关键词: Nitrogen;    Antioxidants;    AsA-GSH cycle;    Oxidative damage;    Salinity;    Triticum aestivum;   

DOI  :  10.1186/s12870-019-2085-3

来源: DOAJ

【 摘 要 】

Abstract Background Salinity is one of the damaging abiotic stress factor. Proper management techniques have been proposed to considerably lower the intensity of salinity on crop growth and productivity. Therefore experiments were conducted to assess the role of improved nitrogen (N) supplementation on the growth and salinity stress tolerance in wheat by analyzing the antioxidants, osmolytes and secondary metabolites. Results Salinity (100 mM NaCl) stress imparted deleterious effects on the chlorophyll and carotenoid synthesis as well as the photosynthetic efficiency. N supplementation resulted in increased photosynthetic rate, stomatal conductance and internal CO2 concentration with effects being much obvious in seedlings treated with higher N dose. Under non-saline conditions at both N levels, protease and lipoxygenase activity reduced significantly reflecting in reduced oxidative damage. Such effects were accompanied by reduced generation of toxic radicals like hydrogen peroxide and superoxide, and lipid peroxidation in N supplemented seedlings. Antioxidant defence system was up-regulated under saline and non-saline growth conditions due to N supplementation leading to protection of major cellular processes like photosynthesis, membrane structure and function, and mineral assimilation. Increased osmolyte and secondary metabolite accumulation, and redox components in N supplemented plants regulated the ROS metabolism and NaCl tolerance by further strengthening the antioxidant mechanisms. Conclusions Findings of present study suggest that N availability regulated the salinity tolerance by reducing Na uptake and strengthening the key tolerance mechanisms.

【 授权许可】

Unknown