Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China)

Computational and Structural Biotechnology Journal

Wenzhen Lin¹  Zhuhua Luo²  Qiancheng Gao³  Zhichao Wang³  Lixing Huang⁴  Yuanhao Gao⁵  Hans-Peter Grossart⁶  Wei Xu⁶  Yu Luo⁶  Ying Guo⁶ 

[1] Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361002, China;Institute of Biochemistry and Biology, Postdam University, Potsdam 14469, Germany;School of Advanced Manufacturing, Fuzhou University, Jinjiang 362200, China;Department of Experimental Limnology, Leibniz Institute of Freshwater Ecology and Inland Fisheries, Stechlin 16775, Germany;Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361002, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China;

关键词: 16S rRNA;    Cfu;    Vibrio diversity;    Vibrio abundance;    Mariculture;    Dongshan bay;   

DOI  :  

来源: DOAJ

【 摘 要 】

The genus Vibrio contains a diverse group of heterotrophic bacteria, which are members of ubiquitous and abundant microbial communities in coastal ecosystems. Vibrio has been frequently found in a wide range of marine environments either by employing Vibrio-specific 16S rRNA sequencing or culturing methods. A combination of molecular and cultivation-dependent methods was developed to more precisely discriminate between different members of the genus Vibrio in seawater. This newly developed assay was subsequently applied to characterize Vibrio community composition in surface water at 18 mariculture sites. It Substantially improved the taxonomic resolution of Vibrio species when compared to traditional 16S rRNA analysis. Our qPCR and cultivation analyses revealed that average Vibrio abundance (Vibrio 16S rRNA gene copy numbers: 3.46 × 106 to 6.70 × 106 copies L−1) and live cell numbers (5.65 × 104–5.75 × 105 cfu mL−1) are significantly related to pH. Total bacteria and Vibrio-specific 16S rRNA metabarcode sequenceing resulted in a total of 10 and 32 operational taxonomic units (OTUs), respectively, and 15 Vibrio species were identified by targeted cultivation of Vibrio strains, with Vibrio fortis and V. brasiliensis dominating in the mariculture areas. The purpose of this study was to combine several analytical methods to improve current sequence-based Vibrio community surveys, and to prove for the effectiveness of this methodological approach comprehensively testing for Vibrio dynamics in different coastal environments.

【 授权许可】

Unknown