Short Chain N-Acyl Homoserine Lactone Production in Tropical Marine Vibrio sinaloensis Strain T47

Sensors

Pui-Wan Tan¹  Wen-Si Tan¹  Nina Yusrina Muhamad Yunos¹  Nur Izzati Mohamad¹  Tan-Guan-Sheng Adrian¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;

关键词: 16S rRNA;    biofilm;    Chromobacterium violaceum CV026;    marine seawater-borne bacteria;    N-acylhomoserine lactone (AHL);    N-butyryl-L-homoserine lactone (C4-HSL);    quorum sensing (QS);   

DOI  :  10.3390/s140712958

来源: mdpi

【 摘 要 】

Quorum sensing (QS), acts as one of the gene regulatory systems that allow bacteria to regulate their physiological activities by sensing the population density with synchronization of the signaling molecules that they produce. Here, we report a marine isolate, namely strain T47, and its unique AHL profile. Strain T47 was identified using 16S rRNA sequence analysis confirming that it is a member of Vibrio closely clustered to Vibrio sinaloensis. The isolated V. sinaloensis strain T47 was confirmed to produce N-butanoyl-L-homoserine lactone (C4-HSL) by using high resolution liquid chromatography tandem mass spectrometry. V. sinaloensis strain T47 also formed biofilms and its biofilm formation could be affected by anti-QS compound (cathechin) suggesting this is a QS-regulated trait in V. sinaloensis strain T47. To our knowledge, this is the first documentation of AHL and biofilm production in V. sinaloensis strain T47.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.