Spatiotemporal Dynamics of Vibrio Communities and Abundance in Dongshan Bay, South of China

Frontiers in Microbiology

Haisheng Chen²  Xiaowan Ma³  Wei Xu⁴  Shuai Yang⁴  LinFeng Gong⁴  Yuanhao Gao⁴  Limei Xu⁴  Zhuhua Luo⁵ 

[1] Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China;Fishery Technology Promotion Station of Dongshan, Zhangzhou, China;Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China;;School of Marine Sciences, Nanjing University of Information Science &

关键词: seasonal pattern;    aquaculture activity;    seawater;    Vibrio diversity;    ecological distribution;   

DOI  :  10.3389/fmicb.2020.575287

来源: DOAJ

【 摘 要 】

The Vibrio genus inhabit estuarine and marine ecosystem throughout the world and can cause severe infections in humans and animals. Previous studies have demonstrated the dynamics of Vibrio at both community and population levels and assessed the close relationship between environmental factors and Vibrio diversity and abundance, such as temperature, salinity, and dissolved oxygen. It is also generally believed that aquaculture is the fastest-growing food sector, which is also applying great environmental impacts on microbial communities in aquatic ecosystems. However, our understanding of the spatiotemporal quantification of Vibrio throughout the four seasons in the aquaculture zone and response to environmental factors remains poor. To explore the spatiotemporal distribution and abundance of the Vibrio community with their related environmental factors and detect the relationships among them, we collected 10 seawater sites spanning four seasons across the whole year in Dongshan Bay for investigating the Vibrio community dynamics. Marked differences in diversity and abundance of the Vibrio community were observed between seasons, which were mainly driven by temperature, dissolved oxygen, nitrate, and nitrite. qPCR analysis showed that Vibrio abundance was most abundant in the summer (5.37 × 106 copies/L), compared with the autumn (4.58 × 106 copies/L), spring (1.18 × 106 copies/L), and winter (1.55 × 104 copies/L). A total of 22 Vibrio operational taxonomic units (OTUs) and 28 species were identified by universal bacteria 16S rRNA gene and cultivation methods, with Vibrio fortis the dominant in these aquaculture areas. To summarize, our present study is one of the few studies to research the occurrence of Vibrio in marine aquaculture of South China, and the results indicate that Vibrio are widely distributed in aquaculture environment and that a further risk assessment is needed to be conducted.

【 授权许可】

Unknown