A Cold-Shock Protein from the South Pole-Dwelling Soil Bacterium Arthrobacter sp. Confers Cold Tolerance to Rice

Genes

Woosuk Cho¹  So Young Kim¹  Xiaoxuan Du¹  Gang-Seob Lee¹  Yeon-Ki Kim²  Kyung Do Kim²  Joung Sug Kim²  Kyong Mi Jun³ 

[1] Biosafety Division, National Institute of Agricultural Sciences, Jeonju 54874, Korea;Department of Biosciences and Bioinformatics, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin 17060, Korea;Genomics Genetics Institute, GreenGene BioTech, Inc., 16-4 Dongbaek jungang-ro 16beon-gil, Giheung-gu, Yongin 17015, Korea;

关键词: cold-shock protein;    Arthrobacter;    rice;    low temperature;    RNA sequencing;   

DOI  :  10.3390/genes12101589

来源: DOAJ

【 摘 要 】

Low temperature is a critical environmental factor restricting the physiology of organisms across kingdoms. In prokaryotes, cold shock induces the expression of various genes and proteins involved in cellular processes. Here, a cold-shock protein (ArCspA) from the South Pole-dwelling soil bacterium Arthrobacter sp. A2-5 was introduced into rice, a monocot model plant species. Four-week-old 35S:ArCspA transgenic rice plants grown in a cold chamber at 4 °C survived for 6 days. Cold stress significantly decreased the chlorophyll content in WT plants after 4 days compared with that in 35S:ArCspA transgenic plants. RNA-seq analysis was performed on WT and 35S:ArCspA transgenic rice with/without cold stress. GO terms such as “response to stress (GO:0006950)”, “response to cold (GO:0009409)”, and “response to heat (GO:0009408)” were significantly enriched among the upregulated genes in the 35S:ArCspA transgenic rice under normal conditions, even without cold-stress treatment. The expression of five cold stress-related genes, Rab16B (Os11g0454200), Rab21 (Os11g0454300), LEA22 (Os01g0702500), ABI5 (Os01 g0859300), and MAPK5 (Os03g0285800), was significantly upregulated in the transgenic rice compared with the WT rice. These results indicate that the ArCspA gene might be involved in the induction of cold-responsive genes and provide cold tolerance.

【 授权许可】

Unknown