Greater Biofilm Formation and Increased Biodegradation of Polyethylene Film by a Microbial Consortium of Arthrobacter sp. and Streptomyces sp.

Microorganisms

卷:8

Chao Fang¹  Zhong-Kui Xie²  Dong Wang³  Yu-Jie Zhong³  Feng-Min Li³  Min Wei³  Fang Han³  Chao-Li Guo³  Xiao-Yan Shi³  Ya-Nan Han³ 

[1] Institute of Ecology, School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China;

[2] Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, No. 320 West Donggang Road, Lanzhou 730000, China;

[3] State Key Laboratory of Grassland Agro-Ecosystems, Institute of Arid Agroecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China;

关键词: Arthrobacter;    carbonyl index;    FTIR spectroscopy;    microbial consortium;    plastic biodegradation;    plastic pollution;   

DOI  :  10.3390/microorganisms8121979

来源: DOAJ

【 摘 要 】

The widespread use of polyethylene (PE) mulch films has led to a significant accumulation of plastic waste in agricultural soils. The biodegradation of plastic waste by microorganisms promises to provide a cost-effective and environmentally-friendly alternative for mitigating soil plastic pollution. A large number of microorganisms capable of degrading PE have been reported, but degradation may be further enhanced by the cooperative activity of multiple microbial species. Here, two novel strains of Arthrobacter sp. and Streptomyces sp. were isolated from agricultural soils and shown to grow with PE film as a sole carbon source. Arthrobacter sp. mainly grew in the suspension phase of the culture, and Streptomyces sp. formed substantial biofilms on the surface of the PE film, indicating that these strains were of different metabolic types and occupied different microenvironments with contrasting nutritional access. Individual strains were able to degrade the PE film to some extent in a 90-day inoculation experiment, as indicated by decreased hydrophobicity, increased carbonyl index and CO₂ evolution, and the formation of biofilms on the film surface. However, a consortium of both strains had a much greater effect on these degradation properties. Together, these results provide new insights into the mechanisms of PE biodegradation by a microbial consortium composed of different types of microbes with possible metabolic complementarities.

【 授权许可】

Unknown