Concurrent Resistance to Carbapenem and Colistin Among Enterobacteriaceae Recovered From Human and Animal Sources in Nigeria Is Associated With Multiple Genetic Mechanisms

Frontiers in Microbiology

Mohammed I. Adah¹  Patrick Butaye²  Patrick Kelly³  Nanven A. Maurice⁴  Paul O. Abba⁵  Salem T. Daniel⁶  Anil Poudel⁷  Olivia Lockyear⁷  Folasade Adekanmbi⁷  Anwar Kalalah⁷  Chengming Wang⁷  Emmanuel O. Ngbede⁸  Chinedu A. Akwuobu⁸  Levi M. Mamfe⁸  Alex A. Adikwu⁹  Andrew M. Adamu^1,10  Yi Yang^1,11 

[1] 0Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Agriculture Makurdi, Makurdi, Nigeria;1Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis;Department of Diagnostics and Extension, National Veterinary Research Institute, Vom, Nigeria;Department of Medical Microbiology and Parasitology, Benue State University Teaching Hospital, Makurdi, Nigeria;Department of Microbiology, College of Sciences, Federal University of Agriculture Makurdi, Makurdi, Nigeria;Department of Pathobiology, Auburn University College of Veterinary Medicine, Auburn, AL, United States;Department of Veterinary Microbiology, College of Veterinary Medicine, Federal University of Agriculture Makurdi, Makurdi, Nigeria;Department of Veterinary Public Health and Preventive Medicine, College of Veterinary Medicine, Federal University of Agriculture Makurdi, Makurdi, Nigeria;Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria;Yangzhou University College of Veterinary Medicine, Yangzhou, China;

关键词: concurrent carbapenem-colistin resistance;    Enterobacteriaceae;    high-risk clones;    Nigeria;    Africa;    whole genome sequencing;   

DOI  :  10.3389/fmicb.2021.740348

来源: DOAJ

【 摘 要 】

Resistance to last resort drugs such as carbapenem and colistin is a serious global health threat. This study investigated carbapenem and colistin resistance in 583 non-duplicate Enterobacteriaceae isolates utilizing phenotypic methods and whole genome sequencing (WGS). Of the 583 isolates recovered from humans, animals and the environment in Nigeria, 18.9% (110/583) were resistant to at least one carbapenem (meropenem, ertapenem, and imipenem) and 9.1% (53/583) exhibited concurrent carbapenem-colistin resistance. The minimum inhibitory concentrations of carbapenem and colistin were 2–32 μg/mL and 8 to >64 μg/mL, respectively. No carbapenem resistant isolates produced carbapenemase nor harbored any known carbapenemase producing genes. WGS supported that concurrent carbapenem-colistin resistance was mediated by novel and previously described alterations in chromosomal efflux regulatory genes, particularly mgrB (M1V) ompC (M1_V24del) ompK37 (I70M, I128M) ramR (M1V), and marR (M1V). In addition, alterations/mutations were detected in the etpA, arnT, ccrB, pmrB in colistin resistant bacteria and ompK36 in carbapenem resistant bacteria. The bacterial isolates were distributed into 37 sequence types and characterized by the presence of internationally recognized high-risk clones. The results indicate that humans and animals in Nigeria may serve as reservoirs and vehicles for the global spread of the isolates. Further studies on antimicrobial resistance in African countries are warranted.

【 授权许可】

Unknown