DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids

eLife

Cristina Soriano-Úbeda¹  Heba Saadeh¹  Elena Ivanova¹  Gavin Kelsey¹  Raquel Romar²  Simon Andrews³  Sebastian Canovas³  Francisco A García-Vázquez³  Soledad García-Martínez³  Pilar Coy³ 

[1] Instituto Murciano de Investigación Biosanitaria, Murcia, Spain;Epigenetics Programme, The Babraham Institute, Cambridge, United Kingdom;Physiology of Reproduction Group, Departamento de Fisiología, Facultad de Veterinaria, Universidad de Murcia-Campus Mare Nostrum, Murcia, Spain;

关键词: In vitro fertilization;    blastocyst;    pig;    epigenetics;    reproductive secretions;    imprinting;   

DOI  :  10.7554/eLife.23670

来源: DOAJ

【 摘 要 】

The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT.

【 授权许可】

Unknown